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shRNA Design Tool



shRNA System:

5' End Site 5' Control Site Loop Sequence 3' Control Site 3' End Site

5' TCTC  NNN...NNN CTTCCTGTCA NNN...NNN  CT     3'
       ||||||||| |||||||||| |||||||||  ||     
3'       NNN...NNN GAAGGACAGT NNN...NNN  GACGTC 5'

Target Length: (17-33 Bases)
Custom Loop Sequence:

References:
Lee, N. et al. (2002) Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20, 500-5.
 
 
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