Using DNA/RNA hybrid oligos from IDT, a group of researchers at Johns Hopkins University have developed a new technique for analyzing RNA in clinical FFPE specimens that offers numerous advantages over traditional methods.
Use of a DNA aptamer to replace monoclonal antibodies in an impedance biosensor that uses microfluidics and a microelectrode for detection of the target molecule, in this case, the H5N1 subtype of avian influenza virus. Aptamer secondary structures and delta G were calculated using the free IDT online UNAFold and OligoAnalyzer programs. Biotinylated aptamers were synthesized by IDT with biotin conjugated at the 5′ end.
Description of a new aptamer selection platform that uses fixed-region blocking elements to ensure library diversity and guard against amplification artifacts. Truncated (random-region sequence only) and full-length library versions of aptamers were synthesized and HPLC-purified by IDT.
Development of the first agent for imaging and quantifying the cancer associated protein, tenascin-C—a tenascin-C-specific single-stranded DNA aptamer. The aptamer was radiolabeled with 18F and 64Cu and used in PET-imaging studies to measure tumor uptake and metabolism. All aptamers, including FITC-aptamers, were synthesized by IDT.
The specificity, uptake, and target binding strength of 2 DNA aptamers were investigated in glioma cells and patient tissue. Aptamers were synthesized by IDT and conjugated at the 5′ end with either Cy® 3 or biotin for purification.
Microfluidic screening of aptamers to the light chain of recombinant Botulinum neurotoxin type A. The DNA library was comprised of sequences containing a central random region of 60 bases flanked by 2 specific 20-base sequences, and was synthesized and purified by IDT.