This paper describes a rapid, cheap, and accessible analytic tool called TIDER (Tracking of Insertions, Deletions and Recombination events) that can be used to quantify incorporation frequency CRISPR-directed mutations. TIDER is derived from the widely used TIDE and the researchers here used the RNP CRISPR approach from IDT.
Researchers at the Foundation for Applied Molecular Evolution (Gainesville, FL, USA) use Ultramer DNA Oligos in experiments demonstrating the practical improvements that can be made to recombinase polymerase amplification (RPA), through addition of self-avoiding molecular recognition systems (SAMRS) to RPA-primers.
Ultramer Oligonucleotides were used to construct calibration standards. Each Ultramer contained a target sequence from one of the transcripts under study. All qPCR assays included a set of 3 Ultramer calibrators.
Ultramer Oligonucleotides were used for BAC mutagenesis via a two-step l–red mediated recombination strategy.
Ultramer Oligonucleotides were used to generate standard curves for absolute quantification. Each Ultramer standard was designed to contain the 5′-end of a transcript under study and qPCR amplified using transcript-specific primers.
Ultramer oligonucleotides were used to construct a new vector that provides one-step incorporation of the PBAD promoter in front of any gene.
Ultramer Oligonucleotides were used as standards due to their purity and quantification precision.
In situ detection of mature microRNAs via labeled extension of Ultramer templates is used to detect human miR-212 expression patterns.
Sets of hybridized, high-fidelity Ultramer Oligonucleotides were assembled into large constructs in a single step. Such constructs are available from IDT as gBlocks® Gene Fragments.