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Cpf1 genome editing using the Alt-R CRISPR-Cpf1 System

The CRISPR-Cpf1 nuclease is the best alternative to the commonly used Cas9 for genome editing. Cpf1 recognizes a protospacer adjacent motif (PAM) sequence of TTTV, which differs from the Cas9 PAM sequence, NGG. Having Cpf1 as a second option increases the likelihood of editing as close as possible to your desired target site. In this presentation, Dr Rolf Turk introduces the optimized Alt-R™ CRISPR-Cpf1 System and explains how it can be used as a powerful new tool for your genome editing research. Dr Turk presents the basics of the system, as well as protocols for getting started in genome editing.

All Genome editing videos

IncreasinggenomeeditingefficiencyandspecificitywithoptimizedCRISPR-Cas9guideRNAs
OvercomingthechallengesofdesigningefficientandspecificCRISPRgRNAs
IncreasinggenomeeditingefficiencywithoptimizedCRISPR-Casenzymes
OptimizedmethodstouseCas9nickasesingenomeediting
HowtoorderAlt-RCRISPRcrRNAinplates
HowtoorderAlt-RCRISPRcrRNAintubes
TipsforeffectiveuseofBLASTandotherNCBItools
NewRNAtoolsforoptimizedCRISPR-Cas9genomeediting
Cpf1genomeeditingusingtheAlt-RCRISPR-Cpf1System
GettingstartedwithCRISPRAreviewofgeneknockoutandhomology-directedrepair
RibonucleoproteindeliveryofCRISPR-Cas9reagentsforincreasedgeneeditingefficiency
Increaseon-targetspecificityofCRISPRgenomeeditingusinganovelhigh-fidelityCas9

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