Amplify libraries for any Illumina sequencing platform
The xGen Library Amplification Primer Mix contains forward and reverse primers complementary to the P5 and P7 sequences of Illumina adapters. These primers are required for post-capture PCR when using xGen Lockdown Probes and Panels for target enrichment. The primer mix can be used to amplify any libraries containing P5/P7 sequences for sequencing on any Illumina platform. Provided as a premixed, liquid formulation, this primer mix is conveniently available in 16, 96, and 192 reaction sizes.
xGen Library Amplification Primer Mix is provided at a concentration of 20 µM for each primer in IDTE buffer (10 mM Tris, 0.1 mM EDTA), pH 8.0. Storage at –15°C to –25°C, with minimal freeze-thaw cycles, is recommended.
This primer mix has been designed to provide optimal post-capture PCR enrichment when using xGen Lockdown Probes and Panels; however, the mix can be used to amplify any NGS library containing adapters with P5 and P7 sequences for Illumina platforms. Follow the manufacturer’s instructions for your chosen library kit when using the primer mix in library amplification.