Harnessing the power of rhAmp PCR
Our proprietary rhAmp PCR technology drives the rhAmpSeq system. Using RNA-base–containing blocked primers (rhAmp primers), this technology harnesses the intrinsic properties of the RNase H2 enzyme to recognize and cleave DNA:RNA duplexes.
rhAmp PCR is thus a powerful tool for increasing amplification specificity and minimizing the biggest limitation of PCR multiplexing—primer dimers.
The rhAmpSeq system combines the performance advantages of rhAmp PCR with a fast, easy-to-use workflow that requires only 2 PCR amplification steps (Figure 1) to generate amplicon libraries for Illumina sequencing platforms. Figure 1 shows both amplification
steps in the rhAmpSeq workflow, highlighting the role of rhAmp PCR technology during the first amplification step (Targeted rhAmp PCR 1). Coupled with our best-in-class DNA and RNA manufacturing, the rhAmpSeq system offers high quality,
targeted, sequencing results quickly and cost-effectively.
To learn more about rhAmp PCR technology, visit this page.