All xGen Blocking Oligos are compatible with any xGen Lockdown Panel or custom xGen Lockdown Probe set. Each oligo is individually synthesized and assessed by ESI mass spectrometry for quality control*.
* With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.
xGen Universal Blockers
IDT currently offers three different ready-to-use, universal blocker reagents. xGen Universal Blockers—TS Mix blocks Illumina LT (p5, p7 – 6
nt and 8
nt) and HT (i5, i7) adapters and is compatible with ligation-based library prep kits, such as Illumina TruSeq kits. xGen Universal Blockers—10 bp TS Mix is compatible with ligation-based library prep kits that use 10 bp index barcodes. xGen
Universal Blockers—NXT Mix contains adapter blockers that are optimized for use with Illumina Nextera library prep kits.
xGen Universal Blockers are offered in 16-, 96-, and 4 x 96-reaction formats for convenient experiment design and for use with xGen Lockdown Panels or xGen Lockdown Probe sets.
Custom xGen Blocking Oligos
Custom blocking oligos can be ordered for specialized applications or other sequencing platforms, and are offered with specified modifications. While we have identified the most commonly used modifications and services required for effective blocking
next generation sequencing, we understand that certain modifications or services may improve the performance of blocking oligo performance for your specific application.
For more information about available blockers, please contact our customer care team at firstname.lastname@example.org.