Figure 1. Consistent Results from xGen® Exome Research Panel. Three users independently performed three capture experiments on different days. Each user prepared DNA libraries according to their preferred method, using well characterized Coriell Cell Repository cell lines. For each capture, 500 ng barcoded library, xGen Universal Blocking Oligos, and 5 µg Cot-1 DNA® were used. Hybridization, washes, PCR amplification, and QC were performed according to the xGen Rapid Capture Protocol v2.1. The data show that the percentage of unique, uniquely mapping, and paired reads mapped to targeted exons (aligned) or also within 150 bp of (flanking) targeted exons (padded) was consistent between users (n = 3 per group; Kruskal-Wallis: aligned p = 0.73, padded p = 0.19). The median percentage of mapped reads on target (aligned) was 83.4%, 86.0%, and 84.1% for Users 1, 2 and 3, respectively. The median percentage of reads mapped to targeted exons and also flanking targeted exons (padded) was 92.4%, 94.1%, and 94.1% for Users 1, 2 and 3, respectively. Bars represent median percent of mapped reads on target. Error bars are S.E.M.