Figure 1. The PACE genotyping system provides improved, cost-effective genotyping compared to the leading industry method, KASP. Genotyping was performed on purified corn DNA samples. Distinct genotyping assays (4) were selected to assess
sequence variability that can affect genotyping outcome. (A) Normal GC/AT content (48% GC). (B) Slightly GC-rich content (68%), (C) AT-rich content (28% GC), and (D) Slighly AT-rich content
(40% GC). master mixes for the PACE and KASP methods were used for genotyping each of the 4 assays (identical primers and samples), in 4 µL reactions, following each manufacturer's protocol. Assay results were analyzed as cluster plots. Genotyping
clusters from the PACE reactions were as well or better defined than the KASP reactions. In addition, the clusters of the PACE reactions were optimal at a fixed number of PCR cycles, whereas with KASP reactions, different cycle numbers were needed.