Nuclease Detection Products

Nucleases are widely present in the laboratory environment and can interfere with many experiments. In particular, single-stranded RNases are ubiquitous, hard to eliminate, and can rapidly degrade important samples used in microarray studies, real-time PCR, northern blots, or cDNA cloning.

IDT has developed reagents that allow for rapid, sensitive detection of RNases and DNases. These reagents are fluorescence-quenched oligonucleotide probes that emit a fluorescence signal only after nuclease degradation. The assay can be read visually or measured and quantified using fluorometry. Assays can be used qualitatively to test lab reagents, equipment, and supplies for nuclease contamination. Assays can be used quantitatively to study enzyme kinetics. Two oligonucleotide substrates are available: one designed to detect RNases and the second designed to detect DNases. The RNaseAlert® substrate employs a FAM™ reporter (Em 520 nm). The DNaseAlert™ substrate employs a HEX™ reporter (Em 555 nm).

Documentation and Support

RNaseAlert® Substrate

DescriptionPart #Pricing
RNaseAlert® Substrate - 25 single use tubes11-04-03-03$75.00 USD
RNaseAlert® Substrate - 2 bulk tubes11-04-02-03$295.00 USD
RNaseAlert® Kit11-02-01-02$110.00 USD
2 ml 10X RNaseAlert® Buffer11-04-02-07$10.00 USD

DNaseAlert™ Substrate

DescriptionPart #Pricing
DNaseAlert™ Substrate - 2 bulk tubes11-04-02-04$245.00 USD
DNaseAlert™ Kit11-02-01-04$90.00 USD
DNaseAlert™ Substrate - 25 single use tubes11-04-03-04$65.00 USD
2 ml 10X DNaseAlert Buffer11-01-02-04$10.00 USD

RNaseAlert and DNaseAlert are available as:

  • Single-use tubes (25 tubes with 0.05 nmole each)—ideal for rapid hand-held visual assays at the research bench
  • Bulk substrate (2 tubes with 2 nmole each)—for use in either visual assays or read in a fluorometer
  • Kit—includes single use tubes, buffer, water, enzyme, and nuclease decontamination solution

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Kit Components:

  • 25 50 pmole Fluorescent Substrate tubes
  • 250 µL RNaseAlert® Buffer
  • 2 mL Nuclease-Free water
  • 50 µL RNase A
  • 50 mL Nuclease Decontamination Solution
  • Kit quality guaranteed for six months from date received when stored at –20°C. 
RNaseAlert

Figure 1. Examination of RNase-Free Sample Versus RNase Positive Control.

RNaseAlert®

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Kit Components:

  • 25 50 pmole Fluorescent Substrate tubes
  • 250 µL DNaseAlert™ Buffer
  • 2 mL Nuclease-Free water
  • 25 µL 2U/mL DNase 1 Enzyme
  • 50 mL Nuclease Decontamination Solution
  • Kit quality guaranteed for six months from date received when stored at –20°C. 

DNaseAlert™

Technical Reports for Download