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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Can IDT make a DNA oligo with a mixture of phosphodiester and phosphorothioate bonds?

Phosphorothioate bonds can be incorporated throughout an oligo by placing an asterisk (*) between the two bases where you would like such modified bonds to appear. As an example, your sequence may look something like this: A*C*T*GCATCAGCCTGCC*A*T*C.

Such oligos must be ordered at 100 nmol scale or higher. Phosphorothioate bonds introduced between the last nucleotides at the 5'- or 3'-end of the oligo inhibit exonuclease degradation.

Custom DNA & RNA

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.