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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How do I resuspend my PrimeTime® qPCR Assay?

PrimeTime qPCR Assays can be re-suspended as 40X, 20X, or 10X stocks. Different concentrations may be preferred depending on the size of the assay and desired final reaction volume. Resuspend the assay in IDTE Buffer (10 mM Tris, 0.1 mM EDTA pH 8.0—available from IDT) at the volumes indicated in the chart below. After adding the volume indicated, pipette the solution up and down the sides of the tube to ensure maximal product recovery. Following resuspension, store at –20°C.aa_resuspending-primetime-assays

Categories:
qPCR & PCR

*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.