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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

What cycling parameters will give me the best results when using the PrimeTime® Gene Expression Master Mix?

The master mix can be used with either standard or fast cycling conditions, depending on what qPCR system you are using. For best results, use one of the cycling parameters provided in the protocol, PrimeTime Gene Expression Master Mix:

  • Standard cycling conditions: 3 min. 95°C; 35–45 x (15 sec. 95°C; 1 min. 60°).
  • Fast cycling conditions: 3 min. 95°C; 35–45 x (5 sec. 95°C; 30 sec. 60°).


*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.