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Frequently Asked Questions

Reading Room

Original IDT Publications, Technical Reports, User Guides and Protocols, MSDS, and other Education Resources

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The qPCR Application Guide provides a general overview, guidance, and troubleshooting advice on the entire qPCR process, from RNA isolation to data analysis. 

You can use the guide to:
  • Review the latest recommendations for qPCR protocols: experimental setup, performance, and analysis
  • Learn specific information regarding the 5′ nuclease assay, including specific resuspension instructions
  • Troubleshoot common problems

Video Library

PrimeTime® qPCR Plates: Overview and Tutorial

Select from predesigned assays for human, mouse, rat, or enter your own primer and probe sequences. Only 24 assays or primer pairs per plate are required, so you can make your own replicate plates for lower cost per reaction.