- Integrated library normalization—Normalase™ technology enables a streamlined library balancing and pooling process without the need to quantify samples
- Customizable—add in additional targets to custom amplicon sequencing panels or predesigned panels
- Time saving—single-tube workflow, go from cDNA to normalized library pool in approximately 3 hours
What is amplicon sequencing?
Targeted DNA sequencing methods, such as amplicon sequencing, provide an opportunity for the interrogation of specific regions of the genome so that researchers can obtain information about the genetic regions they care about the most. In amplicon sequencing,
highly multiplexed PCR generates amplicons of targeted regions of genomic DNA, after which adapters are added via PCR using indexing primers with either combinatorial dual indexes (CDIs) or unique dual indexes (UDIs). The final library fragments
are pooled and sequenced. Index sequences are then used to demultiplex the data bioinformatically, and the sequences of the targeted genomic regions are used for further analysis.
Amplicon sequencing benefits these areas of research:
- Identification of rare variants
- Identification of hot-spot mutations
- CRISPR genome editing confirmation
- Oncology and virology
- Whole genome sequencing confirmation
This highly multiplexed PCR approach provides flexibility for a wide range of experimental designs while helping to reduce costs and workflow time. It is also particularly useful for the discovery of rare somatic mutations in complex samples (e.g., tumors
mixed with germline DNA) and hard-to-sequence areas (e.g., GC-rich regions).
For an in-depth discussion on this method, see our Amplicon sequencing technical overview.
Predesigned and custom amplicon sequencing panels
IDT offers a wide variety of different predesigned panels, but if research takes you in an uncharted direction, custom amplicon sequencing panels can be created for your specific needs.