Do you provide any nonsilencing (negative) controls for Dicer-substrate siRNAs (DsiRNAs)?
We currently offer 2 nonsilencing, negative control DsiRNAs that do not recognize any sequences in human, mouse, or rat transcriptomes, a nontargeting DsiRNA (DS NC1) and a DsiRNA with a scrambled sequence (DS ScrambledNeg).
We strongly recommend using the DS NC1 negative control duplex. This has been extensively bioinformatically screened, and has no activity in human, mouse, and rat cells. The DS ScrambledNeg sequence is an older control that has been kept as a legacy product for customers who have had success with it in their cell lines.