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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

How much fluorescently labeled tracrRNA should I use in my CRISPR-Cas9 experiments?

The amount of Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550 needed varies by application and transfection method. Here are our recommendations. Use these as starting points as you optimize your experiments.

Table. Final concentration of Alt-R® CRISPR-Cas9 tracrRNA – ATTO™ 550 to use in transfection

  FACS analysis Fluorescence microscopy
Lipofection ~10 nM 10 nM
Electroporation ≥0.5 µM
~0.5 µM