FACS-sort cells within 24 hours  after delivery of the ribonucleoprotein (RNP) complex containing fluorescently labeled Alt-R™ CRISPR-Cas9 tracrRNA [ATTO™ 550, ATTO 488, or ATTO 647 (ATTO-TEC, GmbH)]. Data and details on use and function of the fluorescently labeled tracrRNA are included in our Application Note, Fluorescently labeled tracrRNA provides efficient genome editing while allowing cellular microscopy and FACS analysis.
1. Cossarizza A, Chang HD, Radbruch A, et al. Eur J Immunol. 2017;47(10):1584-1797.