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Is a PAM sequence present in every gene?
This is hard to say. Because the frequency of the dinucleotide ‘GG’ is 5.21% in the human genome , it is reasonable to expect that most genes will contain the NGG sequence of the S. pyogenes PAM.
Scherer S Cold Spring Harbor Laboratory. Press., A short guide to the human genome2008, Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press. xiv, 173 p.