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Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.
Why is PAGE purification not available with fluorescent dyes?
We do not recommend PAGE purification for dye labeled oligos due to the presence of ammonium persulfate, a component of the PAGE gel. Ammonium persulfate can break linkages and may damage the ring structures that comprise many fluorescent dyes. At IDT, PAGE gels are exposed to UV light when the oligos are excised from the gel. UV light exposure can also damage fluorescent dyes. HPLC purification provides a pure product without damaging fluorescent modifications.