Nuclease-free reagents for resuspending, diluting, and storing oligos
Our in-house formulated buffers and solutions allow you to confidently work with your oligos. Nucleases are found throughout our natural environment, and since many are used in routine molecular biology applications, they often are found on surfaces within the lab. RNases are difficult to eliminate due to their ubiquitous nature and resistance to heat denaturation. The results of the in-house RNaseAlert, DNaseAlert, and LAL endotoxin testing are provided to you in the Certificate of Analysis documentation.
IDTE (10 mM Tris, 0.1 mM EDTA) is our recommended solution for resuspending and storing single-stranded DNA and RNA oligos. It has been shown to offer the most stability for the longest duration when compared to oligos stored dry or in water. IDTE is available at pH 7.5 or pH 8.0.
Nuclease-Free Duplex Buffer (30 mM HEPES, pH 7.5; 100 mM potassium acetate) is our recommended buffer for storage of duplexed oligos. Like IDTE, it has been shown to offer the most stability for the longest duration when compared to duplexed oligos stored dry or in water.
We do not recommend long-term storage of oligos in water, as this can increase the risk of oligo degradation. However, Nuclease-Free Water is convenient for quick dilutions of storage stocks, or for especially short-term storage.
*RUO—For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.