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DECODED® online newsletter

4 tips for accurate oligonucleotide quantification using Thermo Scientific™ NanoDrop™ instruments

Did you know each oligonucleotide has its own specific conversion factor? Learn how to accurately determine the concentration of your oligonucleotides using Thermo Scientific NanoDrop instruments. Follow these recommendations from the scientists of IDT and Thermo Fisher Scientific.

Custom DNA & RNA

  • Need a library of related DNA or RNA oligo sequences?

  • Tips for minimizing sample misassignment during multiplexed NGS
  • 4 tips for accurate oligonucleotide quantification using Thermo Scientific™ NanoDrop™ instruments
  • Getting enough full-length oligo?
All Custom DNA & RNA articles »

CRISPR genome editing

  • CRISPR-Cas9 mediated HDR: Tips for successful experimental design

  • When and how to use nickases for efficient genome editing
  • Genome editing in cell culture: Isolating single clones for genotypic and phenotypic characterization
  • Electroporation: An alternative to microinjection for creating genetically modified rodents
All CRISPR genome editing articles »

Next generation sequencing

  • NGS target capture recommendations for FFPE samples

  • Minimizing duplicates and obtaining uniform coverage in multiplexed target enrichment sequencing
  • Tips for minimizing sample misassignment during multiplexed NGS
  • Mitigate index hopping while increasing low-frequency variant detection
All Next generation sequencing articles »

Genes & gene fragments

  • Benefits of codon optimization

  • Caffeine addicted bacteria—an iGEM project
  • One-step strategy to create transgenic and KO mouse models (Easi-CRISPR) uses Megamer ssDNA donors and CRISPR RNPs
  • Tips for working with gBlocks Gene Fragments
All Genes & gene fragments articles »

qPCR & PCR

  • Recommended dye combinations for multiplex qPCR

  • Improve genotype calling with simple positive controls
  • Better PCR genotyping—obtain greater precision with RNase H2 activation of assay primers
  • Increase the accuracy and specificity of your genotyping experiments
All qPCR & PCR articles »

Functional genomics

  • Using DsiRNA to map pain pathways in the CNS

  • Guaranteed knockdown of RNA in loss-of-function studies
  • Enzyme-linked DNA aptamer assay provides advantages over standard immunoassays
  • Planning to work with aptamers?
All Functional genomics articles »

Reagents & kits

  • A simple method to detect on-target editing or measure genome editing efficiency in CRISPR experiments

  • Storing oligos: 7 things you should know
  • A simple method to detect on-target editing or measure genome editing efficiency in CRISPR experiments
  • CRISPR guide RNA format affects genome editing outcomes
All Reagents & kits articles »

GMP, OEM & integrations

  • MGB Eclipse Probes for human in vitro diagnostics

  • 4 tips for accurate oligonucleotide quantification using Thermo Scientific™ NanoDrop™ instruments
  • Intact, single cell analysis of protein and nucleic acid biomarkers for clinical diagnostics
  • MGB Eclipse Probes for human in vitro diagnostics
All GMP, OEM & integrations articles »

Optional services

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  • Need a library of related DNA or RNA oligo sequences?
  • Oligo synthesis: Why IDT leads the oligo industry
All Optional services articles »

DECODED online newsletter

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