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Affinity Plus DNA & RNA Oligonucleotides

Oligos incorporating locked nucleic acids for enhanced stability and nuclease resistance

Affinity Plus DNA & RNA Oligonucleotides and Affinity Plus Ultramer DNA Oligonucleotides are custom, single-stranded and duplexed sequences that contain 1–20 locked nucleic acid bases. Incorporation of these modified bases confers increased target specificity and oligo stability. Including Affinity Plus bases in your oligos:

  • Provides an affordable way to improve performance in a variety of hybridization-based applications
  • Allows you to manage sequence Tm, resulting in greater hybridization specificity
  • Improves stability and exonuclease resistance (in vitro and in vivo)

See the Locked nucleic acids technology page for the structure of these modified bases and to learn about the many applications they can facilitate.

Ordering

Affinity Plus Single-stranded DNA

Shipped dry, or resuspended to your specifications.

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Affinity Plus Duplexed DNA

2 oligos, annealed and delivered in a single tube. Shipped dry.

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The following annealing fees will be applied to each duplex ordered:

100 nmol:

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250 nmol:

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1 µmol:

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Affinity Plus Ultramer DNA Oligos

Formulated to 4 nmol or 20 nmol guaranteed yield. Shipped dry or resuspended to 100 µM in IDTE buffer, pH 8.0.

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Affinity Plus Duplexed Ultramer DNA Oligos

2 oligos, annealed and delivered in a single tube. Formulated to 4 nmol or 20 nmol guaranteed yield. Shipped dry.

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Affinity Plus Single-stranded RNA

Shipped dry.

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Affinity Plus Duplexed RNA

2 oligos, annealed and delivered in a single tube. Shipped dry.

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Annealing fees will be applied to duplexed oligos (per duplex), as follows:

100 nmol:

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250 nmol:

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1 µmol:

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Affinity Plus Single-stranded DNA

Shipped dry, or resuspended to your specifications. A minimum of 24 and 96 oligos required for 96- and 384-well plates, respectively.

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Affinity Plus Duplexed DNA

1 oligo pair per well, annealed. Shipped dry. A minimum of 24 and 96 oligo duplexes are required for 96- and 384-well plates, respectively.

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The following annealing fee will be applied to each plate of duplexed DNA:

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Affinity Plus Ultramer DNA Oligos

Shipped dry or resuspended to your specifications. Minimum 24 and 96 oligos required for 96- and 384-well plates, respectively.

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Affinity Plus Duplexed Ultramer DNA Oligos

1 oligo pair per well, annealed. Shipped dry or resuspended in duplex buffer. Minimum of 24 and 96 oligos required for 96- and 384-well plates, respectively.

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The following annealing fee will be applied to each plate of duplexes:

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Affinity Plus Single-stranded RNA

Shipped dry or resuspended to your specifications. A minimum of 24 or 96 oligos required for 96- or 384-well plates, respectively.

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Affinity Plus DNA & RNA Oligonucleotides are single- and double-stranded sequences. With the Oligo Entry ordering tool, you can design the sequence you require with 1 to 20 locked nucleic acid bases to suit your specific research needs.

Affinity Plus Ultramer DNA Oligonucleotides are generated by proprietary synthesis methods that deliver high quality oligos up to 200 bases. They are available single- or double-stranded and can be delivered in tubes or plates.

Every Affinity Plus DNA & RNA Oligo you receive is deprotected and desalted to remove small molecule impurities. In addition, your oligos will be sequence verified via proprietary ESI mass spectrometry methods *, and quantified twice by UV spectrophotometry to provide accurate yield measurements.

*With the exception of mixed base oligos, which could potentially represent multiple sequences and therefore cannot be accurately evaluated by ESI mass spectrometry.

Using the Oligo Entry tool

To enter individual Affinity Plus bases, insert "+" before the base (+A, +C, +G, +T).

Frequently asked questions