Reduce barcode crosstalk in your multiplexed NGS experiments
Quality requirements for synthetic oligos used in
TruGrade oligos are manufactured by proprietary methods that are proven to reduce index cross-talk and increase
With the rapid increased throughput of sequencing platforms, sample multiplexing has become a common method for making economical use of sequencing capacities. However, pooling indexed samples into one sequencing run comes with the risk of sample misidentification. While this can be attributed to many experimental factors, crosstalk during oligo synthesis is a major contributor. TruGrade DNA oligos are manufactured by an exclusive production process that reduces the risk of oligonucleotide crosstalk and, therefore, minimize the risk of barcode misalignment during multiplexing NGS applications.
The TruGrade synthesis and processing service is available on HPLC-purified DNA oligos and Ultramer® DNA oligos. They are available in plates and tubes, and can be formulated to your specifications. TruGrade DNA oligos can be used in a variety of applications including, but not limited to:
Parameter | HPLC purified DNA oligos | Ultramer DNA Oligos |
---|---|---|
Typical cross-contamination1 | 0.10–0.50% | 0.01–0.05% |
Typical time to shipment2 | 5 to 15 business days | 3 to 10 business days |
Synthesis scale | 100 nmol, 250 nmol, or 1 µmol | N/A |
Yield delivered | Variable | 4 nmol or 20 nmol |
1 Typical cross-contamination levels were determined using in-house proprietary QC techniques and information from our customers.
2 Production time depends on the number of oligos ordered. Please inquire for an estimated ship date specific to your order.
Modification type | Modification | DNA oligos (100 nmol, 250 nmol, 1 µmol) | Ultramer DNA Oligos (4 nmol, 20 nmol) |
---|---|---|---|
5′ modifications | 5′ 5-Methyl dC | • | • |
5′ Amino Modifier C12 | – | • | |
5′ Amino Modifier C6 | – | • | |
5′ Biotin | • | • | |
5′ deoxyInosine | • | • | |
5′ deoxyUridine | • | • | |
5′ Phosphorylation | • | • | |
Internal modifications | Int 5-Methyl dC | • | • |
Int deoxyInosine | • | • | |
Int deoxyUridine | • | • | |
Int Spacer 18 | • | • | |
3′ modifications | 3′ Amino Modifier | • | • |
3′ ddC | • | – | |
3′ C3 Spacer | • | – | |
3′ Phosphorylation | • | • | |
Modified bases and bonds | 2′ O-Methyl RNA bases | • | • |
Phosphorothioate Bond | • | • |
Identify oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches
Perform secondary structure analysis
Find the volume needed to dilute your oligo stock to a lower concentration
Find the volume needed to resuspend a dry oligo to a desired concentration