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DNA oligos

Generate consistently reliable data from the highest fidelity oligos available

All single-stranded and duplexed DNA sequences are produced with industry-leading coupling efficiencies, resulting in higher quality DNA products. Our specialized platforms allow us to deliver the purest primers for PCR, dual-labelled probes for qPCR, indexed adapters and fusion primers for sequencing, and a variety of advanced and custom products.

  • Complete confidence in oligos that are verified by ESI-mass spectrometry
  • Begin your project sooner with >90% of orders shipped within 24 hours
  • Unrivalled control of oligo specifications with custom formulation and mixing options

Primer

Ordering

Single-stranded DNA

Shipped dry, or resuspended to your specifications.

RxnReady® Primer Pools

2 oligos premixed in a single tube. Shipped dry, or resuspended to your specifications.

The following mixing fee will be applied to each pool:

Duplexed DNA

2 oligos, annealed and delivered in a single tube. Shipped dry.

The following annealing fees will be applied to each duplex ordered:

100 nmol:
250 nmol:
1 µmol:

Single-stranded DNA

Shipped dry, or resuspended to your specifications. A minimum of 24 and 96 oligos required for 96- and 384-well plates, respectively. Orders for fewer than 288 oligos at the 500 pmol scale will incur a fee.

Primer mix DNA

2 premixed oligos per well. Shipped dry or resuspended to your specifications. A minimum of 24 and 96 mixes is required for 96- and 384-well plates, respectively.

For ordering inquiries, please contact custcare@idtdna.com.

Duplexed DNA

1 oligo pair per well, annealed. Shipped dry. A minimum of 24 and 96 oligo duplexes are required for 96- and 384-well plates, respectively.

The following annealing fee will be applied to each plate of duplexed DNA:

For ordering inquiries, please contact custcare@idtdna.com.

SameDay™ Oligos

Order today and receive your oligos in tubes on the following business day. Shipped dry, or resuspended to 100 µM in IDTE buffer, pH 8.0. Learn more »

HotPlates® Oligo Service

Order today and receive your oligos in 2 business days. Delivered in 96-well, V-bottom plates. A minimum of 48 oligos is required per plate. Shipped dry. Learn more »

The following service fee will be added to each plate:

Rapid HPLC™ Oligos

Order today and receive your HPLC-purified oligos in 2 business days. Shipped dry in tubes. Learn more »

DNA oligos are short, single- and double-stranded synthetic DNA sequences that can be used in nearly any molecular biology application. Using the Oligo Entry ordering tool, you can design your oligos to contain any DNA sequence you require and select from hundreds of modifications to suit your specific research needs.

Every DNA oligo you receive will be deprotected and desalted to remove small molecule impurities. In addition, your oligos will be quality controlled via proprietary ESI-mass spectrometry methods, and quantified twice by UV spectrophotometry to provide accurate yield measurements.

For standard, desalted oligos ≥20 bases, we offer the following yield guarantees:

ProductAvailable lengthGuaranteed yield*
25 nmol DNA oligo15–60 bases3 ODs
100 nmol DNA oligo10–90 bases6 ODs
250 nmol DNA oligo5–100 bases15 ODs
1 µmol DNA oligo5–100 bases50 ODs
5 µmol DNA oligo5–100 bases250 ODs
10 µmol DNA oligo5–100 bases500 ODs
* Guaranteed yield for standard, desalted oligos.

IDT’s long-standing reputation as a leader and pioneer in custom oligo manufacturing is largely due to our vertically integrated proprietary synthesis platforms. Nearly every component of our manufacturing process is designed and developed in-house, including specialized synthesizers that can accommodate the most demanding oligo requests, and high-throughput automation systems that ensure the fastest turnaround times possible. We do not rely on third-party manufacturers for any of the machines or chemical reagents used for synthesis, allowing us to easily monitor and refine instruments and reagents with ease. Our ability to control these variables allows us to produce oligos that are unmatched in quality and consistency.

An important measure of quality for any oligo manufacturer is coupling efficiency, because this directly affects the amount of full-length product you receive. Our manufacturing processes achieve extremely high coupling efficiencies (Figure 1) and are standardized across our global manufacturing sites (Figure 2) to provide you with most consistent quality in the industry. No matter where in the world you are conducting your research, an IDT oligo is an IDT oligo. Our commitment to quality and consistency results in an extremely reliable product that will perform the same each time it is used. Because your research requires a steady supply of oligos over time, this reliability provides confidence in your research.

Figure 1. Proportion of full-length product is a factor of coupling efficiency. DNA oligos from IDT are synthesized on proprietary platforms that deliver higher coupling efficiency than industry standards. The increasing importance of coupling efficiency to increased oligo length is shown.

Figure 2. Daily monitoring of coupling efficiency at all IDT manufacturing sites. Many variables can lead to reduced oligo quality, including ambient temperature, humidity, and input reagents). We track coupling efficiency at all of our manufacturing sites to ensure consistent quality with change in variables. A 30-day rolling average is shown.

OligoAnalyzer™ tool

Identify oligonucleotide properties, including melting temperature, hairpins, dimers and mismatches

UNAFold tool

Perform secondary structure analysis

Dilution Calculator

Find the volume needed to dilute your oligo stock to a lower concentration

Resuspension Calculator

Find the volume needed to resuspend a dry oligo to a desired concentration

The following services represent our most common offerings. If your project requires a service not listed here, contact Technical and Customer Support and our experts will work with you individually to accommodate your request.

Purification

For demanding applications such as multiplex PCR, cloning, mutagenesis, or antisense/RNAi methods, additional purification will significantly improve oligo performance. All purified oligos up to 60 bases receive a purity guarantee via QC by capillary electrophoresis. Learn more »

ProductPAGEHPLCDual PAGE & HPLCIE HPLCRNase-Free HPLCDual HPLC
25 nmole DNA OligoN/AN/AN/AN/AN/AN/A
100 nmole DNA Oligo$50.00 USD$42.00 USDN/A$45.00 USD$75.00 USD$80.00 USD
250 nmole DNA oligo$70.00 USD$69.00 USD$130.00 USD$70.00 USD$105.00 USD$120.00 USD
1 umole DNA Oligo$120.00 USD$105.00 USD$240.00 USD$110.00 USD$160.00 USD$190.00 USD
5 umole DNA Oligo$675.00 USD$240.00 USD$940.00 USD$245.00 USD$340.00 USD$500.00 USD
10 umole DNA Oligo$995.00 USD$370.00 USD$1,450.00 USD$375.00 USD$590.00 USD$760.00 USD

Formulation

Different research needs and various individual preferences in the field require that oligos be delivered in unique formats. With the formulation services listed, you can have your oligos tailor-made to your specific requirements. Learn more »

ProductLabReady (Normalized to 100┬ÁM in IDTE pH 8.0)Normalized Tube Fee
25 nmole DNA Oligo$3.00 USD$5.00 USD
100 nmole DNA Oligo$3.00 USD$5.00 USD
250 nmole DNA oligo$3.00 USD$5.00 USD
1 umole DNA OligoN/A$5.00 USD
5 umole DNA OligoN/A$25.00 USD
10 umole DNA OligoN/A$75.00 USD

Custom quality control

In addition to the free ESI-mass spectrometry analysis provided for all oligos, your project may require one of the analytical services listed below. Learn more »

ProductAnalytical IE-HPLC pH 12.0Analytical RP-HPLCCapillary Electrophoresis Trace
25 nmole DNA Oligo$55.00 USDN/A$45.00 USD
100 nmole DNA Oligo$55.00 USD$55.00 USD$45.00 USD
250 nmole DNA oligo$55.00 USD$55.00 USD$45.00 USD
1 umole DNA Oligo$55.00 USD$55.00 USD$45.00 USD
5 umole DNA Oligo$55.00 USD$55.00 USD$45.00 USD
10 umole DNA Oligo$55.00 USD$55.00 USD$45.00 USD
Description96 well Plate384 well Plate
Entire Plate Capillary Electrophoresis$400.00 USD$1,600.00 USD

Large-scale synthesis

Custom DNA oligos can be ordered in bulk with the same high purity, competitive pricing, and fast turnaround times you expect from IDT. To learn more about our large-scale synthesis capabilities, click here.

GMP and OEM

If you require oligos that are approved for use in molecular diagnostic applications, or if you are interested in our third-party manufacturing services, please click here.