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Frequently asked questions

Our Scientific Applications Support team has assembled a list of frequently asked questions to help you find answers quickly. Filter using one or more categories to focus on specific topics, or use the search bar to perform a text search.

Should I order my gBlocks Gene Fragment with the 5′ phosphate modification?

Adding the 5′ phosphate modification to gBlocks Gene Fragments is unnecessary for most applications.

The 5′ phosphate is required for traditional blunt-end cloning. Do not add 5′ phosphate for Zero Blunt TOPO® cloning (check manufacturer information).