The xGen cfDNA & FFPE DNA Library Prep Kit includes all the reagents required for End Repair, Ligation 1, and Ligation 2 reactions.
Table 1. Specifications, additional reagents, and equipment.
|Feature ||Details |
|Sample types ||High-quality DNA, cfDNA, DNA from FFPE samples, or double-stranded cDNA |
|Input range ||1–250 ng |
|Adapters ||Included in the kit |
|Indexing primers (not included) ||xGen UDI Primers* |
|PCR amplification reagents (not included) ||KAPA Biosystems® HiFi HotStart ReadyMix, or equivalent|
|Compatible sequencing platforms ||Illumina® sequencing instruments |
|Compatible hybrid capture blockers ||xGen Universal Blockers TS|
*Contact us for help ordering other indexing designs or configurations.